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Notification report


General information

Notification Number
B/ES/03/20-CON

Member State to which the notification was sent
Spain

Date of acknowledgement from the Member State Competent Authority
25/02/2003

Title of the Project
Rice for improved yield

Proposed period of release:
15/04/2003 to 31/10/2003

Name of the Institute(s) or Company(ies)
Institut de Recerca i Tecnologia AgroalimentĂ ries (IRTA), Centre de Cabrils
Crta. Cabrils, s/n
08348 Cabrils (Barcelona)
Spain;
CropDesign N.V., Technologiepark 3
B-9052 Zwijnaarde
Belgium;


3. Is the same GMPt release planned elsewhere in the Community?
Yes:


Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
ricepoaceaeoryzaoryza sativaNipponbare

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The gene of interest will alter the morphology of the plants aiming at improving yield parameters. More specifically the gene will alter:

Number of tillers,
Total seed weight,
Total number of seeds.

Also included are a selection marker and a visual marker.

CBI (See Annex)


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The gene of interest originates from rice and its regulatory elements originate from plant species. The gene of interest is an orthologue of (a) gene(s) that is (are) already present in the non-modified rice genome.
The marker genes are from bacterial and animal origin. These organisms are not pathogenic to humans or deleterious to the environment.

CBI (See Annex)


6. Brief description of the method used for the genetic modification:
Rice is modified by using the Agrobacterium mediated transformation technique. Regeneration and selection of modified shoots is on medium containing the selective agent. Transformation is confirmed using the visual marker and by DNA analysis.

Experimental Release

1. Purpose of the release:
The field trial aims at testing the agronomic performance of the modified plants. Special emphasis will be put on yield components (total biomass, plant height, tiller number, harvest index, number of spikes, number of seeds, kernel weight, total seed yield, etc.).
Previous observations in the greenhouse indicate that the transgenic plants as compared to their non-transgenic counterparts do not show changes in survivability, reproduction modus or dissemination, except for the intended traits. It is our intention to carefully observe the behaviour of these plants with respect to survivability, reproduction modus and dissemination in this field trial. Also, there are no indications that the plants will have changed interactions with any organisms or with the environment.
The marker genes have a long history of safe use.


2. Geographical location of the site:
Amposta, (Tarragona)

3. Size of the site (m2):
Four replicates of about 5m2 will be planted from each of the 2 transgenic lines.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Greenhouse trials showed the desired altered plant morphology. No adverse effects were detected.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The introduced genes are not intended to change fertility, seed viability, dormancy or frost sensitivity. In the greenhouse no changes were detected except for those related to yield, as compared to the control plants. If the transgenes show any unusual behaviour in respect to persistence or selective advantage, in the field trial period, this will be recorded.
Rice is a self-pollinator with an estimated cross-pollination frequency of less than 5%. Pollen viability is restricted to a few minutes after release. To prevent out-crossing the plot is isolated from conventional rice fields. Red rice is a wild relative that is sexually compatible with cultured rice.
The incorporated traits are not harmful, directly or indirectly, to humans, animals or other organisms. It is not expected that the modified plants will interact with the environment differently from conventional plants. No specific cultivation, management or harvesting techniques different from conventional practice will be used.


Brief description of any measures taken for the management of risks:
In order to prevent gene transfer to cultured rice the trial will be located at least 30m from any conventional rice field. Pollen transfer occurs only within 10m of the parent plant. Special attention will be paid to eradication of red rice.
Skilled, specialised staff will regularly monitor the plot and record plant development. Seeds will be harvested manually. The vegetal parts will be burned on the plot or shallow incorporated in the soil. The plot will be monitored for re-growth and eventual volunteer plants will be eradicated. In case of an emergency the plants can be destroyed mechanically or by applying herbicides.


Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
28/04/2003 00:00:00
Remarks:
The competent authority for the consent of field trials is Cataluña.