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Notification report


General information

Notification Number
B/DK/05/01

Member State to which the notification was sent
Denmark

Date of acknowledgement from the Member State Competent Authority
24/02/2005

Title of the Project
GENETICALLY MODIFIED ROUND UP READY FODDER BEET (A5/15) IN DANISH FIELD TRIALS

Proposed period of release:
01/04/2005 to 31/12/2009

Name of the Institute(s) or Company(ies)
DLF-Trifolium
Dansk Planteforædling v. Vibeke Meyer, Hojerupvej 31
4660 Store Heddinge;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):
B/BE/93/W20-CON; B/BE/95/WSP4; B/DK/93/02-CON; B/DK/99/02; B/ES/95/02; B/FR/95/01/07-CON; B/FR/96/01/04; B/FR/97/01/06; B/GB/95/R22/1; B/GB/95/R22/2; B/GB/96/R22/8-CON; B/GB/98/R22/12; B/IT/94/18;

Other notifications
CTPS
B/UK/98/R22/17


Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
fodder beetchenopodiaceaebetabeta vulgarisvulgaris var. crassa

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Only one gene, CP4, with regulatory elements is introduced into A5/15. The beet is tolerant to glyphosate due to the expression of CP4-epsps.
No other marker genes or selection genes is present in A5/15.


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
Right Border: A 25 nucleotide sequence that acts as the initial point of DNA transfer into plant cells originally isolated from pTiT37 (0.025Kb).

P-FMV: The 35S promoter from a modified figwort mosaic virus (CoMVb) used to drive expression of CP4 EPSPS and gox genes (0.672 Kb).

AEPSPS/CTP2: The N-terminal chloroplast transit peptide sequence from the Arabidopsis thaliana EPSPS gene (0.310 Kb).

cp4 epsps: The 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) gene from Agrobacterium sp. strain CP4 (1.363 Kb).

E9 3': The 3' end of the pea rbcS E9 gene which provides the polyadenylation sites for the CP4 EPSPS and GUS genes (0.630 Kb).


6. Brief description of the method used for the genetic modification:
Transformation with Agrobacterium tumefaciens with modified plasmid.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable

Experimental Release

1. Purpose of the release:
The purpose of the trials will be to breed , develope and test new fodder beet varieties, to collect information important for risk assesment of the crop and to provide plantmaterial for independent research or general public interest in Denmark.

2. Geographical location of the site:
In 2005 up to five locations in Denmark. Two sites planned:

1. DLF Trifolium, Store Hedddinge: The total area will not exceed 1 ha.
Purpose production of vegetative fodder beet for risk assesment
evaluation.
2. Landbohøjskolen, Højbakkegård. Vegetative beets.
The total area will not exceed 1000m2

Trial locations for years 2006 and 2009 are not defined at time of submission.


3. Size of the site (m2):
See above

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
In the previous 10 year period of releases the risk assesment and potential impacts of growing the beet remain unchanged.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
1. Likelihood of the GMHP becoming more persistent than the recipient or parental plants in agricultural habitats or more invasive in natural habitats.

The introduced glyphosate tolerance trait, could cause increased persistence in agricultural habitats and/or increased invasiveness in natural environments, resulting in an invasive species spreading in the environment, if it occurs. The likelihood of occurrence of this potential adverse effect is negligible. The estimation of the risk posed by the introduced glyphosate tolerance gene is negligible.

2. Any selective advantage or disadvantage conferred to the GMHP

The introduced glyphosate tolerance trait could give competitive advantage of RR fodder beet, resulting in an invasive species spreading in the environment if it occurs. The likelihood of occurrence of this potential adverse effect is negligible. The estimation of the risk posed by the introduced glyphosate tolerance gene is negligible.

3. Potential for gene transfer to the same or other sexually compatible plant species under conditions of planting the GMHP and any selective advantage or disadvantage conferred to those plant species

Sexual compatibility with other fodder beet, allowing cross-pollination and introduced glyphosate tolerance could result in transfer of any selective advantage to other beet plants, which could become invasive and spread in the environment, if it occurs. The likelihood of occurrence of this potential adverse effect is negligible. The estimation of the risk posed is negligible.

4. Potential immediate and/or delayed environmental impact resulting from direct and indirect interactions between RR fodder beet and target organisms (if applicable).

RR fodder beet has no target organisms and, consequently, no relevant characteristics that may cause an adverse environmental effect.

5. Possible immediate and/or delayed environmental impact resulting from direct and indirect interactions of the GMHP with non-target organisms, (also taking into account organisms which interact with target organisms), including impact on population levels of competitors, herbivores, symbionts (where applicable), parasites and pathogens.

Potential exposure of non-target organisms in the receiving environment to the CP4 EPSPS protein, expressed in RR fodder beet could result in direct toxicity of the expressed protein or indirect population effects. The likelihood of occurrence of this potential adverse effect is negligible. The estimation of the risk posed by the potential adverse effect is negligible.

6. Possible immediate and/or delayed effects on human health resulting from potential direct and indirect interactions of the GMHP and persons working with, coming into contact with or in the vicinity of the GMHP release(s).

Potential for an interaction of the CP4 EPSPS protein with persons handling RR fodder beet gives a potential for adverse occupational health effects, resulting from toxicity or allergenicity of the expressed protein. The likelihood of occurrence of this potential adverse effect is negligible. The estimation of the risk posed by the potential adverse effect is negligible.

7. Possible immediate and/or delayed effects on animal health and consequences for the feed/food chain, resulting from consumption of the GMO, if it is intended to be used as animal feed.

Expression of the CP4 EPSPS protein might have a potential for adverse health effects in the feed/food chain or potential effects on wholesomeness, resulting from toxicity or allergenicity of the CP4 EPSPS protein. The likelihood of occurrence of this potential adverse effect is negligible. The estimation of the risk posed by the potential adverse effect is negligible.

8. Possible immediate and/or delayed effects on biogeochemical processes resulting from potential direct and indirect interactions of the GMO and target and non-target organisms in the vicinity of the GMO release(s).

Interaction of the CP4 EPSPS protein, expressed in RR fodder beet, with non-target organisms involved in biogeochemical processes might have a potential for adverse effects to nutrient cycles in the soil, resulting from potential toxicity of the expressed protein. The likelihood of occurrence of this potential adverse effect is negligible. The estimation of the risk posed by the potential adverse effect is negligible.

9. Possible immediate and/or delayed, direct and indirect environmental impacts of the specific cultivation, management and harvesting techniques used for the GMHP where these are different from those used for non-GMHPs.

RR fodder beet does not require specific cultivation, management or harvesting techniques and, therefore, has no relevant potentially harmful characteristics.


Brief description of any measures taken for the management of risks:
Areas with sown/planted Roundup tolerant beet, will be clearly labelled in the field as well as in fieldplans.

Planting with flowering beet: The flowering beets will be isolated from other flowering wild or cultivated beets by a distance of minimum 1000m, and by sowing of a belt of pollen isolation to reduce the spread of pollen.
Any volunteer bolters in or near the planting , will be roughed.

Trials with vegetative beet: Plant starting to bolter, will be roughed before flowering, to avoid spread of pollen. Bolters on surrounding areas will be controlled.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable at this moment.

Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known