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Notification report


General information

Notification Number
B/DE/10/209

Member State to which the notification was sent
Germany

Date of acknowledgement from the Member State Competent Authority
03/09/2010

Title of the Project
Evaluation of the resistance of KP4 bearing genetically modified spring wheat plants against loose smut in the field with special consideration of effects on resistance against other fungal pathogens

Proposed period of release:
01/04/2011 to 31/10/2013

Name of the Institute(s) or Company(ies)
University of Rostock, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
spring wheatpoaceaetriticumtriticum aestivumGolin and Greina

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Genes for kp4 (viral protein 4 of a double strand RNA virus, which parasitizes Ustilago maydis), bar (from Streptomyces hygroscopicus which confers herbicide resistance against Phosphinothricin) and bla (encodes for a beta-lactamase and confers resistance against the antibioticum ampicillin) were introduced into wheat under control of different promoters.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
KP4: under control of the ubiquitin promoter (of the uidA gene from Zea mays) to drive expression of kp4 gene, kp4 gene codes for the viral protein KP4 of a double strand RNA virus, which parasitizes Ustilago maydis, t35S terminator (of Cauliflower mosaic virus (CaMV)) transcription terminator of the kp4 gene; actin promoter (of Oryza sativa) to drive expression of bar gene, bar gene from Streptomyces hygroscopicus which confers herbicide resistance against Phosphinothricin (Bialaphos) to transformed cells, t35S terminator (of CaMV) transcription terminator of the bar gene. The bla gene and fragments of them (codes for a beta-lactamase and confers resistance against the antibioticum ampicillin) are under control of a bacterial promoter. The bla gene in this case is without function and had been transmitted during the transformation process.

6. Brief description of the method used for the genetic modification:
The transformation of wheat embryos by particle bombardment was performed according to Finer et al. 1992. The plant regeneration was performed by an optimized method according to Clausen et al. 2000. Two spring wheat cultivars from Switzerland (Greina and Golin) were used for the transformation.

Finer, J.J. Vain, P., Jones, M.W., Mcmullen, M.D. 1992: Development of the particle inflow gun for DNA Delivery to plant cells. Plant Cell Reports 11: 323-328
Clausen, M., Krauter,R., Schachermayr, G., Potrykus, I., Sautter, C.: Antifungal activity of a virally encoded gene in transgenic wheat. Nat. Biotechnol. 18: 446-449


7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
not applicable

Experimental Release

1. Purpose of the release:
Evaluation of the new technological properties and ingredients of the genetically modified wheat with focus of
- behaviour of the transgenes in field grown wheat
- environmental impact of transgene expression
- secondary growth behaviour
- resistance against loose smut
- resistance against other fungal pathogens


2. Geographical location of the site:
Thulendorf (Mecklenburg/Vorpommern)
Ausleben/Üplingen (Sachsen Anhalt)


3. Size of the site (m2):
2011: 108 m2 for each release site
2012: 108 m2 for each release site
2013: 108 m2 for each release site


4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
not applicable

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
There is no scientific reason to assume that the modifications by biosynthesis of the viral protein KP4 may lead to changes in reproduction, dispersion, persistence or invasiveness of these plants compared to conventional wheat plants. On basis of current experience the transgenic plants do not differ from non-transgenic plants in growth, size, phenology and seed formation. Under outdoor conditions there is no selective pressure that might lead to a selective advantage of any of the transgenic lines. Due to current experience no effect of the KP4 protein in the plants on pest organisms other than members of the order Ustilaginales is expected. In the previous field trials no differences in plant colonization by pest and beneficial organisms between transgenic and near isogenic lines have been detected, nevertheless in order to assure this data of effects of the KP4 on other fungal pathogens will again be investigated in this project.

Brief description of any measures taken for the management of risks:
The measures for risk control are laid down in a monitoring plan. In order to avoid cross-pollination, the distance of the nearest cultivation of wheat plants will be at least 50 m. Seed bearing spikes will be collected manual, analyzed, removed and autoclaved. Sowing and harvesting machinery will be cleaned on site to prevent the dispersal of GM seed. Harvested plant material will be transported from the site in closed and labelled containers to the laboratories for analyses. After harvesting the spikes, the cornstalks will be steam-heated to 250 °C for 2 hours. The steam-heated plant material will be chopped and worked into the soil. The area will be controlled for volunteers for a period of one consecutive year. During the release period the Field Manager and trained personnel will monitor the trial site at defined intervals

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
The field trials are designed to investigate the environmental impacts:
- whether the new features change the volunteer characteristics of the plants
- whether there are any effects on resistance against other fungal pathogens


Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
16/03/2011 00:00:00
Remarks: