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Notification report


General information

Notification Number
B/DE/08/202

Member State to which the notification was sent
Germany

Date of acknowledgement from the Member State Competent Authority
01/12/2008

Title of the Project
Changes in environmental behaviour of cyanophycin producing transgenic potato plants.

Proposed period of release:
01/06/2009 to 30/06/2011

Name of the Institute(s) or Company(ies)
University of Rostock, Agrar- und Umweltwissenschaftliche Fakultät
Agrobiotechnologie
Justus-von-Liebig-Weg 8
Universität Rostock
18059 Rostock;


3. Is the same GMPt release planned elsewhere in the Community?
Yes:
Germany;

Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):
B/DE/05/176; B/DE/08/199;

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosumAlbatros

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Genes for PsbY-cphATe (Cyanophycin Synthetase) of T. elongates (linked to chloroplast transit peptide from A. thaliana) and/ or nptII (neomycin phosphotransferase II gene of E.coli) were introduced into potato under control of the constitutive 35S promoter or the tuberspecific B33 promoter from the patatin gene from potato.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
PsbY-cphATe – gene:
The constitutive 35S promoter (of CaMV) is used to drive expression of PsbY-cphATe gene. The PsbY-cphATe gene (of Thermosynecococcus elongatus BP-1, linked to the DNA encoding PsbY chloroplast transit peptide isolated from Arabidopsis thaliana) codes for the cyanophycin synthetase which is transported to the chloroplast and produces the copolymer of L-aspartic and L-arginine. The t35S terminator (of CaMV) is used to eterminate transcription of the PsbY-cphATe gene. The constitutive 35S promoter (of CaMV) is used to drive expression of nptII gene. The nptII gene confers kanamycin resistance to transformed cells. Transcription of the nptII gene is terminated by the t35S terminator (of Cauliflower mosaic virus).

B33-PsbY-cphATe – gene:
The tuber specific B33 promoter (from patatin gene from Solanum tuberosum) is used to drive expression of the PsbY-cphATe gene. The PsbY-cphATe gene (of Thermosynecococcus elongatus BP-1, linked to the DNA encoding PsbY chloroplast transit peptide isolated from Arabidopsis thaliana) codes for the cyanophycin synthetase which is transported to the chloroplast and produces the copolymer of L-aspartic and L-arginine. The t35S terminator (of CaMV) is used to terminate transcription of the PsbY-cphATe gene. The constitutive 35S promoter (of CaMV) is used to drive expression of nptII gene. The nptII gene confers kanamycin resistance to transformed cells. Transcription of the nptII gene is terminated by the t35S terminator (of Cauliflower mosaic virus).

Neomycin Phosphotransferase II:
The constitutive 35S promoter (of CaMV) drives expression of nptII gene. The nptII gene from Escherichia coli confers kanamycin resistance to transformed cells. The transcription of the nptII gene is terminated by the t35S terminator (of CaMV).


6. Brief description of the method used for the genetic modification:
An Agrobacterium-mediated transformation and plant regeneration system slightly modified according to HORSCH et al. (1985) was used for the transformation of the potato cultivar Albatros.

Horsch, R., Fry, J., Hoffmann, N.L., Eichholtz, D., Rogers, S.G., Fraley, R.T. (1985) A simple and general method of transferring genes into plants. Science 227: 1229-1231


7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
Evaluation of environmental behaviour of the genetically modified potatoes with focus on
- Possible changes in frost resistance
- Possible changes in the interaction of transgenic plants and soil
microbes
- Rotting velocity, intensity and degradation products
- Monitoring of secondary growth
- Analysis of soil parameters during the rotting process


2. Geographical location of the site:
Thulendorf (Mecklenburg/Vorpommern)

3. Size of the site (m2):
a) 750 m2 for propagation purpose during the vegetation periods 2009 and 2010 to produce enough tuber material for the experiments.

b) 64 m2 for experimental purpose during the winter periods 2009/2010 and 2010/2011.


4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
In B/DE/05/176, genetically modified potatoes showed no effects on environmental and human health.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
There is no scientific reason to assume that the production of Cyanophycin may lead to changes in reproduction, dispersion, persistence or invasiveness of these plants compared to conventional potato plants. On basis of current experience the transgenic plants do not differ from non-transgenic plants in growth, size, phenotype and seed formation. Under field conditions there is no selective pressure that might lead to a selective advantage of any of the transgenic lines. Due to current experience no effect of the proteins in the plants on pest or beneficial organisms or on health and environment are expected.

Brief description of any measures taken for the management of risks:
The areas used for the experiments and for the propagation of tuber material will be controlled differently. The measures for changes in frost resistance, rotting and secondary growth are timed during the winter season from October to May. Therefore, flowering or vegetative propagation is not expected. Additionally, the tubers will be buried in special bags to increase the recovery rate to 100 %. The area will be controlled for volunteers for a period of one consecutive year.
The measures for risk control on the area used for propagation of tuber material are laid down in a monitoring plan. Sowing and harvesting machinery will be cleaned on site to prevent the dispersal of GM seed. Harvested plant material will be transported from the site in closed and labelled containers to the laboratories for analyses. Before harvest haulm will be inactivated chemically and ploughed in soil. The area will be controlled for volunteers for a period of one consecutive year. During the release period the Field Manager and trained personnel will monitor the trial site at defined intervals.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
The field trials are designed to investigate the environmental impacts:

- whether the cyanophycin production changes frost resistance of the
plants
- whether a normal rotting of the transgenics after the winter season is
ensured
- that no accumulation of Cyanophycin occurs in the soil
- that soil microbes are not affected by rotting of Cyanophycin producing
potatoes


Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
03/06/2009 00:00:00
Remarks: