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Notification report


General information

Notification Number
B/DE/07/195

Member State to which the notification was sent
Germany

Date of acknowledgement from the Member State Competent Authority
23/01/2008

Title of the Project
In-field evaluation of loose smut resistance in genetically modified spring wheat expressing KP4 protein with special focus on effects of resistance against other fungal pathogens.

Proposed period of release:
01/06/2008 to 31/10/2010

Name of the Institute(s) or Company(ies)
University of Rostock, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
wheatpoaceaetriticumtriticum aestivumGolin and Greina

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
kp4-gene (viral protein 4 of a double strand RNA-virus infecting the fungus Ustilago maydis), bar gene (from Streptomyces hygroscopicus confering herbicide resistance against phosphinothricine) and bla gene (codes for a beta-lactamase and confers resistance against the antibioticum ampicillin) were introduced into wheat under control of different promoters (see 4.).

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The kp4-gene: expression of the kp4 gene is under control of the ubiquitin promoter of the uidA gene from Zea mays and the t35S terminator of CaMV. The kp4 gene codes for the viral protein KP4 of a double strand RNA-virus, which infects the fungus Ustilago maydis. It confers resistance against certain fungi.
The bar gene: expression is controlled by the actin promoter of Oryza sativa and the t35S terminator of CaMV. The bar-gene derives from Streptomyces hygroscopicus and confers herbicide resistance against phosphinothricine (Bialaphos). The gene was used as a transformation marker.
The bla-Gene codes for a beta-lactamase and confers resistance against the antibioticum ampicillin. The bla-Gene and fragments thereof are under control of a bacterial promoter. Therefore, the bla-gene has been transmitted during the transformation process, but is non functional in planta.


6. Brief description of the method used for the genetic modification:
The transformation of wheat embryones by particle bombardement was performed according to Finer et al. 1992. The plant regeneration was performed by an optimized method according to Clausen et al. 2000. Two spring wheat cultivars from Switzerland (Greina and Golin) were used for transformation.

Finer, J.J., Vain, P., Jones, M.W., McMullen, M.D. (1992) Development of the particle inflow gun for DNA delivery into plant cells. Plant Cell Reports 11: 323-328
Clausen, M., Krauter, R., Schachermayr, G., Potrykus, I., Sautter, C. (2000) Antifungal activity of a virally encoded gene in transgenic wheat. Nat. Biotechnol. 18:446-449


7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
Evaluation of agronomic properties and transgene expression of the genetically modified wheat with focus on

- behaviour of the transgenes in field grown wheat
- environmental impact of transgene expression
- secondary growth behaviour
- resistance against loose smut
- resistance against other fungal pathogens.


2. Geographical location of the site:
Thulendorf (Mecklenburg/Vorpommern)
Ausleben/√úplingen (Sachsen Anhalt)


3. Size of the site (m2):
72 m2 of genetically modified wheat for each release site and year, respectively.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Not applicable.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
There is no scientific reason to assume that modifications induced by biosynthesis of the viral protein KP4 may lead to changes in reproduction, dispersal, persistence or invasiveness of these plants compared to non modified wheat. On the basis of current experience the transgenic plants do not differ from non-transgenic plants in growth, size, phenology and seed formation. At out door conditions, there is no selective pressure that might lead to a selective advantage of any of the transgenic lines. Due to current experience no effect of the novel proteins on pest or beneficial organisms or on health and environment is expected except the intended effect on certain fungal pathogens.

Brief description of any measures taken for the management of risks:
The measures for risk control are laid down in a control sceme: In order to avoid cross-pollination, the distance of the nearest cultivation of wheat plants will be at least 50 m. Seed bearing spikes will be collected manually, analyzed, removed and autoclaved.
Sowing and harvesting equipment will be cleaned on site to prevent the dispersal of GM seed. Harvested plant material will be transported from the site in closed and labelled containers to the laboratories for analyses. After harvesting the spikes, the cornstalkes will be mowed, collected and disposed of at a biogas plant site in the immediate vicinity. Vegetative and non reproductive plant material will be chopped and worked into the soil. The release site will be controlled for volunteers in the year following the release. In case of monitored transgenic wheat volunteers, the control will be prolonged for another year. During the release period the field release manager and trained personnel will monitor the trial site at defined intervals.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
The field trials are designed to investigate on the following environmental impacts:

- whether the new features change the volunteer characteristics of the plants
- whether there are any effects on resistance against other fungal pathogens


Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
13/05/2008 00:00:00
Remarks: