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Notification report


General information

Notification Number
B/DE/06/178

Member State to which the notification was sent
Germany

Date of acknowledgement from the Member State Competent Authority
03/08/2006

Title of the Project
Increase of grain protein content in winter wheat

Proposed period of release:
01/10/2006 to 31/08/2008

Name of the Institute(s) or Company(ies)
Leibniz-Institut of Plant Genetics and Crop Plant Research (IPK), ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
wheatpoaceaetriticumtriticum aestivumCerto

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Triticum aestivum (cv. Certo) has been ballistically transformed. The transgenic winter wheat plants (termed basis lines) contain: a barley-derived sucrose transporter (HvSUT1) under control of the hordein B1 promoter of barley (line HOSUT), a Vicia faba-derived amino acid permease under control of the 1Ax1 promoter of wheat (line XAP) and under control of the HvSUT1 promotor (line SUTAP). The lines contain the marker genes phosphinotricin acyl transferase (bar)conferring resistance against glufosinate-ammonium (HOSUT, XAP, SUTAP) and beta-lactamase conferring resistance against ampicillin (XAP, SUTAP). All lines could putatively contain the cat-gene conferring resistance against chloramphenicol but were not tested for. At green house conditions, expression of the transporter genes in winter wheat plants increases the grain protein content up to 30%. In addition, lines XAP and SUTAP show an early flowering phenotype. These transgenic basis lines were crossed into seven non transgenically modified winter wheat lines from a breeding company. After crossing, 816 individually different homozygous transgenic lines were developed containing one of the described target genes (wheat breeding nursery). Influence of transgene expression on grain composition and flowering time of the breeding nursery lines was not analysed up to now.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
1.
Name: 1Ax1-Promotor
Function: regulatory subunit of seed storage protein Ax2
Source: Triticum aestivum var. Cheyenne
Length of nucleotide/
amino acid sequence: 2376 bp (Pos. 1…2376)
Accession/Reference: M22208.2; GI:14043051

2.
Name: Hordein-Promotor
Function: regulatory subunit of seed storage protein hordein B1
Source: Hordeum vulgare var. Carlsberg II
Length of nucleotide/
amino acid sequence: 550 bp (Pos. 1...550)
Accession/Reference: X87232.1; GI:809030
3.
Name: HvSUT1-Promotor
Function: regulatory subunit of sucrose transporters 1 (sut1)
Source: Hordeum vulgare subsp. vulgare
Length of nucleotide/
amino acid sequence: 1445 bp (Pos. 1...1445)
Accession/Reference: Wang Q. (2003)

4.
Name: VfAAP1
Function: Amino acid permease 1 (aap1)
Source: Vicia faba var. minor
Length of nucleotide/
amino acid sequence: 1427 bp (Pos. 39...1466)
Accession/Reference: AJ318809; GI:15216025

5.
Name: VfAAP1
Function: Amino acid permease 1 (AAP1)
Source: Vicia faba var. minor
Length of nucleotide/
amino acid sequence: 475 AS
Accession/Reference: CAC51423; GI:15216026

6.
Name: HvSUT1
Function: Sucrose transporter 1 (sut1)
Source: Hordeum vulgare subsp. vulgare
Length of nucleotide/
amino acid sequence: 2044 bp (Pos. 1...2044)
Accession/Reference: AJ272309.1; GI:7024414

7.
Name: HvSUT1
Function: Sucrose transporter 1 (SUT1)
Source: Hordeum vulgare subsp. vulgare
Length of nucleotide/
amino acid sequence: 523 AS
Accession/Reference: CAB75882.1; GI:7024415

8.
Name: Hordein terminator
Function: Terminator of seed storage protein hordein B1
Source: Hordeum vulgare var. Carlsberg II
Length of nucleotide/
amino acid sequence: 1660 bp (Pos. 1...1660)
Accession/Reference: Brandt A. (1985)

9.
Name: OCS terminator
Function: Terminator of Octopin synthetase
Source: Agrobacterium tumefaciens
Length of nucleotide/
amino acid sequence: 727 bp (Pos. 1...727)
Accession/Reference: AF242881.1; GI:8572673


6. Brief description of the method used for the genetic modification:
not applicable

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
not applicable

Experimental Release

1. Purpose of the release:
Expression of transport proteins aims at increasing of sink strength of the developing winter wheat grains. The transgenic lines show at green house conditions an up to 30% increase of the grain protein content and early flowering phenotype. The two new traits are of high agronomical relevance. Verification of the results by growing of the transgenic plants at outdoor environmental conditions and combination with traits being additionally relevant for breeders (as represented by the conventional winter wheat lines from the breeding company) may result in new agriculturally useful breeding lines.

2. Geographical location of the site:
Gatersleben, Saxonia-Anhalt, Germany

3. Size of the site (m2):
Transgenic base lines: 150 m2

Transgenic breeding nursery: 1,050 m2 (35 m x 30 m)


4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
not applicable

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The transgenic plants confer resistance against the herbicide BASTA. However, every herbicide different from BASTA can be used to destroy the transgenic winter wheat plants if necessary. No significant environmental benefit can be derived from the transgenes integrated.

Brief description of any measures taken for the management of risks:
The release area will be surrounded by Phacelia at a width of 5 m to isolate the site; at least 20 m of distance will be maintained to any agriculturally used area, pollen dispersal is prevented by using winter wheat as transgenic crop as wheat is largely self-fertilising; seed dispersal is prevented by using of bird-scaring equipment and field spanning nets during grain ripening; the release area is monitored during the following year.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
not applicable

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
23/11/2006 00:00:00
Remarks: