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Notification report


General information

Notification Number
B/DE/05/171

Member State to which the notification was sent
Germany

Date of acknowledgement from the Member State Competent Authority
10/11/2005

Title of the Project
Development of a standardised method to minimise out crossing of transgenic oilseed rape in field experiments

Proposed period of release:
01/04/2006 to 31/10/2007

Name of the Institute(s) or Company(ies)
Verein zur Förderung Innovativer und nachhaltiger AgroBiotechnologie MV - FINAB e.V., ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
spring oilseed rapebrassicaceaebrassicabrassica napusnapus (syn. oleifera)Drakkar, Lisora

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Genes for either stilbensynthase (VST1), and/or suppression cassettes (sense – spacer – antisense) for the sinapic acid glycolsyl transferase (SGT) and/or sinapoylglucose cholin sinapoyl transferase (SCT) genes were introduced into spring oilseed rape in order to either synthesise the health-improving compound resveratrol and/or to reduce the phenylic compound sinapine. Either the neomycin phosphotransferase II gene (nptII) or the phosphinothricine acetyltransferase gene (bar) each under control of the 35S promoter have been used as selectable marker gene.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The genes of interest are VST I (stilbensynthase) taken from Vitis vinifera, and suppression cassettes for the SGT (sinapic acid glycolsy transferase) and SCT (sinapoylglucose cholin sinapoyl transferase) genes, each taken from Brassica napus. The genes are used alone and in combination.
These genes of interest are regulated by the napin promoter from Brassica napus.
The terminators used are:
- an endogeneous terminator of Vitis vinifera,
- the nos terminator from A. tumefaciens,
- the 35S terminator from Cauliflower mosaic virus.
As selectable marker genes the neomycin phosphotransferase gene (npt II) or the, phosphinothricine acetyltransferase gene (bar) from Streptomyces hygroscopicus, each flanked by the 35S promoter and 35S terminator from cauliflower mosaic virus, have been used.


6. Brief description of the method used for the genetic modification:
An Agrobacterium-mediated transformation and plant regeneration system slightly modified according to DE BLOCK et al. (1989) was used for the transformation of spring oilseed rape cultivars Drakkar and Lisora.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
Evaluation of trait performance under field conditions and assessment of various methods to minimise out crossing of transgenic oilseed rape in release trials.

2. Geographical location of the site:
Sanitz (Mecklenburg-Vorpommern)

3. Size of the site (m2):
Total area of genetically modified oilseed rape will not exceed 480 m2.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Not applicable.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
There is no scientific reason to assume that the modifications by biosynthesis of resveratrol glucoside and/or reduction of sinapine content may lead to changes in reproduction, dispersion, persistence or invasiveness of these plants compared to conventional oilseed rape. On basis of current experience the transgenic plants do not differ from non-transgenic plants in growth, size, phenology and seed formation. There is no known selection pressure in favour for or against the presence of resveratrol glucoside or low sinapine or a combination of both characters in seeds. Due to current experience no effect of the resveratrol glucoside in the seeds on pest or beneficial organisms or on health and environment is expected.

Brief description of any measures taken for the management of risks:
In order to minimise cross-pollination, transgenic field plots are surrounded by either fertile conventional and/or male-sterile oilseed rape. The distance of the nearest cultivation of oilseed rape plants will be at least 1,5 km.
Sowing and harvesting machinery will be cleaned on site to prevent the dispersal of GM seed. Harvested seed material will be transported from the site in closed and labelled containers to the laboratories for analyses. Remaining seed will be inactivated. Vegetative plant material will be chopped and worked into the soil. The area will be controlled for volunteers for a period of 4 consecutive years. During the release period the Field Manager and trained personnel will monitor the trial site at defined intervals.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
The field trials are designed to investigate the pollen flow influenced by different kinds of protective borders and to find the optimal method to minimise transgenic pollen flow.

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
10/05/2006 00:00:00
Remarks: