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Notification report


General information

Notification Number
B/DE/05/166

Member State to which the notification was sent
Germany

Date of acknowledgement from the Member State Competent Authority
17/08/2005

Title of the Project
Influences of growth of transgenic potatoes on soil quality in fields.

Proposed period of release:
01/05/2006 to 31/10/2007

Name of the Institute(s) or Company(ies)
Technische Universität München, Center for Life and Food Sciences, Chair of Plant Breeding, D-85350 Freising, Germany.;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosumbaltica

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The amount of the endogenous zeaxanthinepoxydase (ZEP) was reduced in potato tubers by sense (clone SR 48/00#17) and antisense (clone SR 47/00#18) expression of a fragment of the potato zep gene under the control of the globular starch synthase (GBSS) promoter. ZEP oxidises zeaxanthin to violoxanthin. After reduction of its activity in the transgenic lines the amount of the carotenoid zeaxanthin increased. For selection of the transgenic clones the nptII marker gene was used.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The constituent zep fragment has a length of 1044 bp. The zep fragment encodes a part of the zeaxanthin epoxidase which catalyses the conversion of zeaxanthine to violaxanthin.The zep fragment was isolated by PCR from S. tuberosum cv. Desiree and ligated into the TA cloning vector pCR2.1. From there it was transferred by BamHI/Xbal-restriction into the vector pPGB121S. pPGB121S was constructed by cloning a 0,8kb promoter fragment of GBSS into pBI101 which derives from the binary vector pBIN19. Two constructs were built that way. In one, zep was linked in sense, in the other zep was linked in antisense-orientation to the GBSS promoter resulting in pPGBzep and pPGBzep-antisense, respectively.

6. Brief description of the method used for the genetic modification:
For transformation Agrobacterium tumefaciens strain LBA 4404 was used. The selection of transformed tissue was performed on kanamycin containing medium.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
This project focusses on biological safety research, namely on the influence of the transgenic potatoes on agricultural soil organisms. In addition, plants and tubers produced will be tested on whether they are identical to wildtype plants in characters other than the zeaxanthin content. Furthermore, it will be examined to which extent pollen of the modified plants will fertilize other potato cultivars.

2. Geographical location of the site:
The experiment will be performed on two locations in Upper Bavaria:

1. TUM experimental station Roggenstein, 82223 Olching, district Fürstenfeldbruck, Flurstück 740/5.
2. A farm site at Oberviehhausen, 94574 Wallerfing, district Deggendorf, Flurstück 1458


3. Size of the site (m2):
Area with transgenic plants: 324 m²

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
In a previous release (B/DE/02/135) during a three years period (2003-2005) using the same transgenic lines no effects on the environment were observed. No effects on human health were monitored either.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Potato has no sexually compatible relatives in Germany. Thus, an unexpected distribution in the environment is not possible. Furthermore, potatoes are vegetatively propagated. Seedlings from volunteer seeds will be destroyed by frost. There is no obvious advantage in terms of persistence, invasiveness or distribution been mediated by the transgene.

Brief description of any measures taken for the management of risks:
1. The whole area is fenced and marked by respective signs.
2. Cleaning of the planting and harvesting tools on the experimental plot.
3. Planting of 15m Sinapis arvensis around the experiment.
4. No other potatoes in a distance of 850 m.
5. Picking of those flowers from the transgenic plants where no berries are to be harvested for quality tests and seed production (for measuring outcrossing rates).
6. Non-used plants and tubers are macerated on the experimental plot or autoclaved.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
The experiments shall give answers to the following questions:

1. Influence on microbial populations in the rhizosphere and residuesphere.
2. Influence of the transgenic characters on other metabolites in different plant organs.
3. Influence on a rotation with winter wheat.
4. Influence on the degradation by microbes after growth of the transgenic potatoes.
5. Comparison of N metabolism of transgenics and controls.
6. Estimation of outcrossing rates to neighbouring rows in the experimental plots.


Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
30/08/2006 00:00:00
Remarks:
Consent given for the release at "Oberviehhausen" on 30/05/06 and for the release at "Roggenstein" on 30/08/06