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Notification report


General information

Notification Number
B/DE/02/145

Member State to which the notification was sent
Germany

Date of acknowledgement from the Member State Competent Authority
06/11/2002

Title of the Project
"Phytoremediation of soils contaminated with heavy metals by transgenic poplars" (Phytosanierung von Schwermetallen in Böden mit Hilfe gentechnisch veränderter Pappeln: Stabilität des Transgens und Einfluss auf die Rhizoflora, Verbundprojekt Spezifische Umweltwirkungen transgener Gehölze, Teilprojekt 5, Sicherheitsforschung und Monitoring im Programm der Bundesregierung: Biotechnologie 2000)

Proposed period of release:
01/01/2003 to 31/12/2005

Name of the Institute(s) or Company(ies)
Albert-Ludwigs-Universität Freiburg, Fahnenbergplatz, D-79085 Freiburg/Breisgau, Germany;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):
B/DE/01/133;

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
poplarsalicaceaepopuluspopulus deltoidesINRA Klon 717 1-B4, female

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
nptII: Kanamycin- resistance
gshI from E.coli: over-expression of Gamma-Glutamylcystein Synthetase, two- to fourfold higher glutathione concentration


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
right border from Agrobacterium tumefaciens (161 bp)
Nopalin Synthase Promotor from Agrobacterium tumefaciens (306 bp)
npt-II Gen from Tn5 from E. coli (983 bp) - Kanamycin resistance
Nopalin Synthase Terminator from Agrobacterium tumefaciens (255 bp)
Sequenz from Gen III (441 bp) no relevant function
lacI Gen (141 bp) no relevant function
lacZ Gen + Polylinker (116 bp) no relevant function
35S Terminator from Cauliflower-Mosaic-Virus (725 bp)
gshI Gen from E.coli (1585 bp, Anlage D) - -glutamylcysteine synthetase
35S-Promotor with P70 double-Enhancer from Cauliflower-Mosaic-Virus (750 bp)
lacZ Gen + Polylinker (113 bp)
M13ori (300 bp) no relevant function
lacI Gen (130 bp) no relevant function
left Border from Agrobacterium tumefaciens (147 bp)


6. Brief description of the method used for the genetic modification:
Transformation with Agrobacterium tumefaciens

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
The used trees are female plants, about a half year old. The duration of release will be restricted to three years, while first flowering is expected after seven to eight years. Therefore, generative dissemination of the transgenic poplars can be excluded. In order to control a vegetative spread of the transgenic plants during the release and two years afterwards, the areas will be controlled and root suckers removed.

Experimental Release

1. Purpose of the release:
Phytoremediation of soils, gene stability, biological safety monitoring

2. Geographical location of the site:
1.) Helbra, Flur 4, field 5/68 (11°29’18’’ / 51°33’22’’)
2.) Großörner, Flur 4, field 278/1 (11°29’19’’ / 51°37’06’’)
both sites: rural district Mansfelder Land, Saxonia-Anhalt, Germany


3. Size of the site (m2):
2 x 2500 m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
no risk for human health and environment are expected

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Since the GMPTs do not express any toxic substances, no impact on the environment is expected.

Brief description of any measures taken for the management of risks:
If premature flowers will be detected, these plants will be removed. During the release and two years afterwards, the areas will be controlled and root suckers will be removed.
Mycorhiza fungi and soil bacteria will be monitored for the presence of the transgene.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
not applicable

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Not known