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Notification report


General information

Notification Number
B/DE/02/135

Member State to which the notification was sent
Germany

Date of acknowledgement from the Member State Competent Authority
29/11/2002

Title of the Project
Improvement of health quality aspects of food through increase and modification of carotinoid content.
Verbesserung der gesundheitlichen Qualität von Lebensmitteln durch Erhöhung und Modifikation des Carotinoid-Gehaltes


Proposed period of release:
01/04/2003 to 31/10/2005

Name of the Institute(s) or Company(ies)
Technische Universität München
Wissenschaftszentrum Weihenstephan
Lehrstuhl für Pflanzenbau und Pflanzenzüchtung, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosumBaltica

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The Zeaxanthin-potatoes are potatoes with a modified carotinoid composition. The carotinoid content of potatoes is generally low, while Lutein (ca. 45%) and Violaxanthin (ca. 40%) are the dominating carotins. In the Zeaxanthin-potatoes the synthesis of Violaxanthin is genetically modified to result in the accumulation of Zeaxanthin instead of Violaxanthin. This is achieved by inactivation of the Zeaxanthinepoxydase by co-suppression (clone SR 47/00#17 and SR 47/00#18 with pPGB-zep_sens construct in sense orientation) or antisense technique (clone SR 48/00#17 and SR 48/00#20 with construct pPGB-zep_anti ins antisense orientation).

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
T-DNA of pPGBzep_sense:
(name source function)
1. pBIN19-sequence
Agrobacterium tumefaciens T37 (Bevan 1984)
Right border of Ti-plasmid; promotor Nopalinsynthase; Kanamycinresistance npt II Tn5; Nopalinsynthas-terminator; M13mp19 sequence with fragment of the alpha- peptide of the beta Galactosidase
2. GBSS- Promotor
Solanum tuberosum
Promotor for tuber specific expression
3. pBIN19-sequence
Vector
PUC19 polylinker of Vector pBIN19
4. zep-fragment
Solanum tuberosum
pCR2.1-polylinker (Invitrogen); zep_sens-pcr-fragment; pCR2.1- polylinker
5. pBIN19-sequence
Vector
PUC19 polylinker of Vector pBIN19
6. pBI101-sequence
Vector (Jefferson et al.1987)
Nopalinsynthase-terminator
7. pBIN19-sequence
Vector
3`fragment of the alpha-peptide of the beta-Galatosidase; M13mp19 intergenic zone; left border of pTiT37


T-DNA of pPGBzep_antisense:
(name source function)
1. pBIN19-sequence
Agrobacterium tumefaciens T37
Right border of Ti-plasmid; promotor Nopalinsynthase; Kanamycinresistance npt II Tn5; Nopalinsynthas-terminator; M13mp19 sequence with fragment of the alpha- peptide of the beta Galactosidase
2. GBSS-Promotor
Solanum tuberosum
Promotor for tuber specific expression
3. pBIN19-sequence
Vector
PUC19 polylinker of Vector pBIN19
4. zep-fragment
Solanum tuberosum
pCR2.1-polylinker (Invitrogen); zep_antisense-pcr-fragment; pCR2.1- polylinker
5. pBIN19-sequence
Vector
PUC19 polylinker of Vector pBIN19
6. pBI101-sequence
Vector
Nopalinsynthase-terminator
7. pBIN19-sequence
Vector
3`fragment of the alpha-peptide of the beta-Galactosidase; M13mp19 intergenic zone; leftborder of pTiT37


6. Brief description of the method used for the genetic modification:
Leaf and stem sections of in-vitro cultured Solanum tuberosum var. Baltica were incubated with Agrobacterium tumefaciens LBA4404 over night. Sprouts were regenerated on Kanamycin containing media for selection of transformed cells. Sprout were re-cultured three times every 14 days and checked for persisting agrobacteria.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
not applicable

Experimental Release

1. Purpose of the release:
Production of tubers for analysis of nutrition physiology. Besides the production of tubers comparisons of agronomic performance and traits between transgenics and wild types will be conducted. Tests on herbicide effectiveness will be performed for the preparation of possible control of weed potatoes. Investigations on the sexual transfer of the trangenic trait to nearby grown potato cultivars will be performed by analysis of the developed seeds.

2. Geographical location of the site:
Place: Roggenstein
Municipality: Emmering
Postal code: 82223
Administrative district: Fürstenfeldbruck
Country: Bayern
Realty (Flurstück): 740/5 Gemarkung Olching
Elevation: 510 o.s.l.


3. Size of the site (m2):
5400 m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
not applicable

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
An elevated persistence in agricultural habitats or an elevated invasion of natural habitats, would imply that the fertility of seeds, or the frost tolerance of the potato tubers are altered by the genetic modification. Such effects are not to be expected. A transfer of genes to wild relative species is impossible since the occurring species Solanum dulcamara and Solanum nigrum are sexually not compatible to Solanum tuberosum.
It is not to be expected that the transgenic potatoes interact in a basically different way with other organisms (e.g. pathogens or pests) as compared to non-transgenic potatoes. Due to the genetic modification the potatoes do not produce new metabolites, but the composition of carotinoids is altered. Qualitative new trait of the transgenic plants could not be observed. Therefore impacts on the environment are not expected.


Brief description of any measures taken for the management of risks:
A genetransfer by pollen occurs only within a distance of 10 m. The minimal distance to the next conventional potato field will be 50 m. A pollen transfer to wild species is not possible (s.o.).
Before maturity the above ground parts of the plants will be destroyed mechanically and chemically to prevent the production of viable seeds. The harvest will be performed half-mechanical and by hand. Potentially remaining tubers will most probably not survive the winter since the plot will not be ploughed before winter. In the following seasons the plot will be cultivated with crop species that allow a easy monitoring for potato plants. Potentially occurring potato plants will be destroyed.
Further storage and processing of the harvested tubers will be done in facilities with safety level S1 permission. Remaining potato haulm will be burned on the field. Any other remaining plant material (leafs or tiny tubers) will be shallow incorporated with a rotary hoe according to common agricultural practise. The plot will be monitored for re-growing potatoes for the following two years.
The trial will be regularly monitored by the staff which is also responsible for plant protection applications. Diseased plant will be removed and destroyed. On a 2-3 weeks basis the plot will be monitored by the scientist in charge and the plant development will be recorded.
In case of an unintended multiplication the transgenic plants can be destroyed at any time by herbicides or mechanically. Potentially growing potato seedlings in the following seasons can be removed by ploughing or with herbicides.
The trial can be stopped at any time by application of herbicides or a rotary hoe.


Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
28/03/2003 00:00:00
Remarks: