General informationNotification NumberB/CZ/11/2Member State to which the notification was sentCzech RepublicDate of acknowledgement from the Member State Competent Authority15/02/2011Title of the ProjectDeliberate release of genetically modified SCLW-GP-PHYA barley producing enzyme phytaseProposed period of release:01/04/2011 to 30/09/2015Name of the Institute(s) or Company(ies)Institute of Experimental Botany AS CR, ;
3. Is the same GMPt release planned elsewhere in the Community?NoHas the same GMPt been notified elsewhere by the same notifier?NoGenetically modified plantComplete name of the recipient or parental plant(s)
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:Spring barley SCLW-GP-PHYA has been genetically modified to produce a fungal phytase gene (phyA) from Aspergillus niger. The enzyme 3-phytase is effectively releasing phosphorus and cations which are bound to phytic acid in barley seeds.
|Common Name||Family Name||Genus||Species||Subspecies||Cultivar/breeding line|
In addition, all transgenic lines have also integrated a selection gene (bar) that confers herbicide resistance, and luciferase reporter gene (luc). These traits have been used during in vitro culture steps to screen for genetically modified cells.Genetic modification3. Type of genetic modification:Insertion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:SCLW-GP-PHYA barley has been genetically modified by insertion of the fungal phytase gene from Aspergillus niger. Cereals (including barley), oil crops and legumes contain phytic acid, myo-inositolhexakisphosphate that is an antinutritional compound. Ions of iron and cations (manganese, magnesium, zinc and calcium) which are bound to phytic acid are difficult to digest. These complexes negatively affect the usability of phosphorus and bound cations in human and monogastric animal foodstuffs. Monogastric animals (e.g. pigs, poultry, rabbits and fish) have the digestive tract with very low phytase activity that does not enable them to decompose phytates. Phytic acid is not digested by these animals, which is a main cause of surface water pollution with phosphates in the areas with intensive livestock units (pigs and poultry). Therefore, the feed for monogastric animals is supplemented with microbial phytase at the amount ensuring high phytate degradation. An effective way of eliminating antinutritional effect is overproduction of endogenous phytase in barley grain.
Zygotic immature embryos of spring barley were co-transformed by particle bombardment with combinations of two vectors:
The first vector contains the gene phyA from Aspergillus niger, the second vector contains the herbicide selection gene (bar) from Streptomyces hygroscopicus, conferring resistance against the plant herbicide Basta, and the common luciferase reporter gene (luc) from Photinus pyralis.
Transgenic plants were regenerated from embryogenic callus selected on Bialaphos containing medium, and analyzed for presence phyA, bar, luc genes by PCR and RT-PCR.
The higher-phytase lines show 3.5 fold increase content of pytase activity in barley grain.
Genetic elements contained in the vectors:
phyA Gene, fungal 3-phytase from Aspergillus niger
α-amylase promoter, promoter region from Hordeum vulgare cv. Himalaya synthase gene
bar gene, phosphinothricin acetyltransferase coding region from Streptomyces hygroscopicus, herbicide resistance
ubi promoter, promoter region from Zea mays polyubiquitin gene
luc gene, reporter gene from the (Photinus pyralis) for detecting gene expression
ubi promoter, promoter region from Zea mays polyubiquitin gene6. Brief description of the method used for the genetic modification:SCLW-GP-PHYA barley has been co-transformed by particle bombardment transformation method (biolistic transformation).7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:Not applicableExperimental Release1. Purpose of the release:The purpose of the trialing program is to verify stability of the transgene phyA in progeny.2. Geographical location of the site:Experimental station Lukavec, region Vysocina3. Size of the site (m2):Maximum 1000 m, including non-modified border rows.4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:The transgenic plants have been cultivated in GMO greenhouse, plants appeared normal in all respects.Environmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:No risks to human and animal health or the environment from the deliberate release of genetically modified SCLW-GP-PHYA barley, with higher activity of endogenous phytase in barley grain, are expected as per information contained in the environmental risk assessment included in the notification.Brief description of any measures taken for the management of risks:Barley is mainly self pollinated plant with cleistogamic flowers. The shedding of pollen from the genetically modified plants will be controlled by maintaining a 100-meter isolation distance with any other non-experimental barley crop. In addition, the trial site will be surrounded by eight border rows of conventional barley of a similar maturity that will be also destroyed at the end of the release.
Grains are fixed on a cob and enclosed in many husks that protect the seeds from outside contact. Thus seed dispersal of individual kernels is not likely to occur.
No plant or plant product originating from the trials will enter the food or feed chains.
After the release, the plot will be regularly visited during the following year in order to ensure removal of barley volunteers, if any. Although volunteer barley cannot generally survive hard winter, barley volunteers, if any, will be monitored in order to ensure their destruction.
There will be no commercial barley grown on the same area of the field the following year.Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:Not applicableFinal report-European Commission administrative informationConsent given by the Member State Competent Authority:Yes27/05/2011 00:00:00Remarks:The consent was on 07/05/2012 extended to 31/12/2016 and to the site of Palacky University in Olomouc.