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Notification report


General information

Notification Number
B/CZ/10/1


This notification has been withdrawn by the notifier
19/01/2011


Member State to which the notification was sent
Czech Republic

Date of acknowledgement from the Member State Competent Authority
30/09/2010

Title of the Project
Field trials with glyphosate tolerant H7-1 sugar beets in the Czech Republic

Proposed period of release:
01/01/2011 to 31/12/2013

Name of the Institute(s) or Company(ies)
KWS Osiva s.r.o., KWS Osiva s.r.o., on behalf of KWS SAAT AG, and Reparsky institut, spol. s r.o.;


3. Is the same GMPt release planned elsewhere in the Community?
Yes:


Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):
B/BE/00/VSP2; B/DE/07/192; B/ES/10/01; B/FR/00/07/01; B/FR/99/01/07; B/FR/99/11/02; B/IT/99/03; B/IT/99/27; B/IT/99/36;

Other notifications

Russia, North and South America


Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
sugar beetchenopodiaceaebetabeta vulgarisvulgaris var. saccharifera

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
RR sugar beet event H7-1 contains a fully functional and intact gene encoding the CP4 EPSPS protein, which confers tolerance to glyphosate, the active ingredient in Roundup herbicides.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
Right Border: A 21-25 bp nucleotide sequence that acts as the initial point of
DNA transfer into plant cells originally isolated from A. tumefaciens pTiT37.
P-FMV: The 35 S promoter from a modified Figwort Mosaic Virus (FMV).
ctp2: The N-terminal chloroplast transit peptide sequence from the
Arabidopsis thaliana EPSPS gene.
cp4 epsps: The 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS)
gene from Agrobacterium sp. strain CP4.
E9 3’: The 3’ end of the Pisum sativum rbcS E9 gene, containing
polyadenylation sites that direct mRNA processing and polyadenylation.
Left Border: A 21-25 bp nucleotide sequence that delimits the T-DNA transfer
into plant cells, originally isolated from A. tumefaciens plasmid
pTi15955, a derivative of the octopine type plasmid pTiA6.


6. Brief description of the method used for the genetic modification:
A disarmed Agrobacterium tumefaciens plant transformation system was used to produce event H7-1. This delivery system is well documented to transfer and stably integrate transferred DNA (T-DNA) into the plant nuclear chromosome. The vector used is PVBVGT08. The original transformation was conducting using a diploid fertile sugar beet line.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable

Experimental Release

1. Purpose of the release:
- Acquisition and evaluation of agronomic properties, e.g. susceptibility to plant pests and diseases, susceptibility to abiotic stressors and germination characteristic of the H7-1 sugar beet;
- Assessment of the efficiency of different weed control systems;
- Selectivity, efficacy and residue studies (e.g. for herbicide registration);
- Acquisition of phenotypic characteristics of the H7-1 sugar beet during the growing season;
- Compositional analysis and evaluation of leaves and beets, yield performance and processing quality of the beets.


2. Geographical location of the site:
Bylany (region Pardubicky)
Straskov (region Ustecky)


3. Size of the site (m2):
3 500 m2 per site and year will be planted with H7-1 sugar beet.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
No change in dormancy or characteristics other than glyphosate tolerance could be observed in the used seed lots comparing H7-1 and common used, diploid, multicarp and monocarp sugar beet hybrids.
It can be concluded that there are no meaningful differences between H7-1 and the nontransgenic counterparts in regards to survivability.


Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Analysis of the characteristics of sugar beet event H7-1 have shown that the likelihood of potential adverse effects on human health and the environment in the European Union, resulting from its cultivation and use as any other sugar beet is negligible. Therefore, the overall environmental risk posed by the proposed field trials with event H7-1 is also negligible.

Brief description of any measures taken for the management of risks:
The trial site will be checked regularly during the period of the deliberate release to remove bolting sugar beet plants before pollen emission.
The trial plots will be encircled by 5 m or 12 rows of non-modified variety. The plants from these rows will be harvested and handled as GM material.
The plants will be chopped in the field.
Plant material remaining after harvest will be destroyed and will not be used for human food or animal feed. The sites will be monitored for one year after the release and any volunteer sugar beet appearing will be eliminated before flowering. During this year, sugar beet will not be grown on the trial sites.
In case any adverse environmental effects, linked to the deliberate release of H7-1 sugar beet, are observed during the period of release, these will be reported immediately to the Competent Authorities.
At the end of the field-testing, a report of will be made available by the notifier to the Competent Authorities. This report will detail any unexpected adverse environmental effects that were observed during the general surveillance, if any, and further actions elicited as an effect of these findings, if applicable.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable.

Final report
-

European Commission administrative information

Consent given by the Member State Competent Authority:
No
01/01/1900 00:00:00
Remarks:
Notification withdrawn by the notifier