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Notification report


General information

Notification Number
B/CZ/09/02

Member State to which the notification was sent
Czech Republic

Date of acknowledgement from the Member State Competent Authority
15/01/2009

Title of the Project
Release into the environment of genetically modified potatoes with the change of late blight resistance

Proposed period of release:
01/05/2009 to 30/09/2013

Name of the Institute(s) or Company(ies)
Vesa Velhartice, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosum

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
- the resistance to potato late blight was changed due to protease inhibitor (SPI2)
- nptII gene (tolerance to kanamycin) as selectable marker gene to identify transgenic cells in tissue culture


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
- T-DNA borders, pTiT37, for incorporation into plant chromosome
- nptII gene, E. coli, kanamycin tolerance in plant material
- Promoter and terminator from nopaline synthase gene, A. tumefaciens, gene regulation
- Promoter of CAMV p35S, gene regulation, and terminator from octopine synthase gene, A. tumefaciens, gene regulation
- SPI2:GFP gene, Galleria mellonella, protease inhibitor in fusion with GFP
- Promoter of CAMV p35S, gene regulation, and terminator from nopaline synthase gene, A. tumefaciens, gene regulation
- SPI2-His6 gene, Galleria mellonella, protease inhibitor with histidine tag


6. Brief description of the method used for the genetic modification:
The purpose of the release is, to compile data on agronomical performance, as well as to compare late blight resistance with control plants, as well as to generate seed tubers.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable

Experimental Release

1. Purpose of the release:
The purpose of the release is, to compile data on agronomical performance, as well as to compare late blight resistance with control plants, as well as to generate seed tubers.

2. Geographical location of the site:
Kolinec, Plzensky region

3. Size of the site (m2):
Less than 1 ha.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
There has been previous release of potato lines transformed for potato late blight resistance in Czech Republic (BASF). No adverse impacts on the environment or human health have been recorded in the trials.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The genetically modified potato lines show in tests in vitro decrease or increase in potato late blight resistance. No difference regarding persistence in agricultural habitats or invasiveness in natural habitats compared to conventional potato varieties is expected. Nor is it expected that a selective advantage or disadvantage is conferred to the GM lines via the introduced traits. Due to the measures to be taken during the release (distance to or absence of cultivated and wild relatives) will the potential for gene transfer virtually be excluded. Interactions of the GM potato lines with non-target species and resulting effects will be comparable to those of conventional potato varieties. No toxic or allergenic effects are expected from protease inhibitor (potato produces many other protease inhibitors), nor from the neomycin phosphotransferase. Measures in place under current field trial practice will protect the trial from damage by animals as well as safeguard that all seed and plant material is properly managed, harvested, stored, transported or disposed of to minimize or prevent contact to humans or animals. No effects are expected on biogeochemical processes. The GM potato lines will be cultivated under conventional agricultural practices (one part of the plants will be not treated against Phytophtora infestans infection).

Brief description of any measures taken for the management of risks:
An isolation distance of 10 m to other potato varieties will be observed. Planting and harvesting equipment will be cleaned on site to prevent the dispersal of GM tubers. There will be no potato cultivation on the release area two years following the release. Potential volunteers will be monitored and removed according to conventional agricultural practice. During the release the trial site will be monitored at defined intervals.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not appropriate

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
12/05/2009 00:00:00
Remarks: