General informationNotification NumberB/CZ/08/02Member State to which the notification was sentCzech RepublicDate of acknowledgement from the Member State Competent Authority31/03/2008Title of the ProjectExperimental release of genetically modified tobacco into the environmentProposed period of release:01/05/2008 to 01/09/2018Name of the Institute(s) or Company(ies)Charles University in Prague, Faculty of Science;
3. Is the same GMPt release planned elsewhere in the Community?NoHas the same GMPt been notified elsewhere by the same notifier?NoGenetically modified plantComplete name of the recipient or parental plant(s)
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:Plants of Nicotiana tabacum were transformed with cdc25 gene from Schizosaccharomyces pombe under constitutive promotor 35S CaMV, together with a nptII gene for resistance to kanamycine. The products of cdc25 gene is a mitotic activator in S. pombe which acts through dephosphorylation of CDK/cyclin complex.Genetic modification3. Type of genetic modification:Insertion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:Introduced gene: cdc25 gene from Schizosaccharomyces pombe. The expression of the gene is expected to induce premature entry of cells into mitosis; neomycinephosphotransferase gene (nptII) from Escherichia coli - kanamycine resistance, serves as a selection marker.6. Brief description of the method used for the genetic modification:Cdc25 cDNA clone, pCDC25-S9, was isolated from Schizosaccharomyces pombe. The chimaeric construct was made by inserting the coding region of cdc25 between CaMV 35S promoter and nopalin synthase termination sequence. The chimeric gene was inserted into binary vector pBIN19 and electroporated into Agrobacterium tumefaciens strain LBA4404 (Bell et al. 1993), which was used to transform tobacco leaf discs by a method described by Horsch et al. (1985)7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:NAExperimental Release1. Purpose of the release:Transformed tobacco plants will be used for experimental purpose only. The aim of the cultivation is physiological and morphological evaluation of the effect of cdc25 expression, possibly causing premature entry of plant cells into mitosis.2. Geographical location of the site:Experimental plot in the area of Department of Plant Physiology, Faculty of Science, Charles University in Prague3. Size of the site (m2):25 m24. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:There was no previous release conducted with this modified plant.
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Transformed plants of Nicotiana tabacum cv. Samsun were repeatedly cultivated in the experimental greenhouse. No negative impact on human health or environment was observed.Environmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:The introduced gene is not expected to increase selective advantage of the plants and to be accompanied with any kind of environmental risks.Brief description of any measures taken for the management of risks:The spreading of pollen and seeds will be prevented by harvesting the experimental plants before flower opening and seed formation. The tobacco plants have no special organs suitable for vegetative multiplication. Survival of plants after harvest and their spreading is not expected. Nevertheless, the experimental plot and its surrounding will be monitored one month after harvest and at the beginning of the following growing season.Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:No field trials are planned. The cultivation will be restricted to small scale experiments on the plots in the area of Department of Plant Physiology. The aim of the cultivation is physiological and morphological study of the effect of premature entry of plant cells into mitosis.Final report-European Commission administrative informationConsent given by the Member State Competent Authority:Yes15/07/2008 00:00:00Remarks: